GeoMicrobiology (EAS/BIOL 6765)

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Geochemistry

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 I. General Information

Dr. Thomas J. DiChristina; office: 1240 ES&T; phone: 4-8419; e-mail: thomas.dichristina@biology.gatech.edu.
Dr. Martial Taillefert; office: 1238 ES&T; phone: 4-6043; e-mail: mtaillef@eas.gatech.edu.
Classes: ES&T L1116 Tu, Th 15:05-16:25

II. Objectives

The objective of this course is to describe interactions between microorganisms and the geosphere to bridge the gap between geochemistry and environmental microbiology. Fundamental processes such as: microbial physiology and genetics; geochemical controls on microbial diversity and activity; microbiological controls on geochemical reaction networks; redox and acid-base geochemistry; biogeochemical cycles; and evolution will be studied.

III. Course Material

Course material is based on class notes, handouts, and assigned readings. There are no specific textbook associated with this course, but the following will be used regularly:

· T. Fenchel; G. M. King; T. H. Blackburn. 2000. Bacterial Biogeochemistry: the ecophysiology of mineral cycling. Academic Press.
· E. A. Paul; F. E. Clark. 2000. Soil Microbiology and Biochemistry. Academic Press.
· J. F. Banfield; K. H. Nealson. 1997. Geomicrobiology: interactions between microbes and minerals. Reviews in Mineralogy Vol. 35. Mineralogical Society of America.
· F. J. Stevenson; M. A. Cole. 1999. Cycles of Soils: Carbon, Nitrogen, Phosphorus, Sulfur, Micronutrients, 2nd Edition. Wiley.
· M.T. Madigan; J.M. Martinko; J. Parker. 2000. Brock Biology of Microorganisms. 9th edition, Prentice Hall.
· J.W. Lengeler, G. Drews, H.G. Schlegel. 1999. Biology of the Prokaryotes, Blackwell Science.
· W. Stumm; J. J. Morgan. 1996. Aquatic Chemistry. Chemical equilibria and rates in natural waters. Wiley.

IV. Grading

Paper presentations: 50%. Term paper: 40%. Participation: 10%.

V. Course Outline

1. Introduction
    The geochemist’s view of the world; the microbiologist’s view of the world.
2. Carbon fixation: origin of life
    Cyanobacteria and photosynthesis; stromatolites; limiting nutrients; unculturable and culturable photosynthesizers, methanogenesis.
3. Carbon oxidation: greenhouse gases
    Mineralization of organic matter; inhibition/competition between organisms; greenhouse gases; gas hydrates and methane oxidation.
4. Nitrogen cycle: a complex redox network
    Nitrogen chemistry; mechanisms and regulation of bacterial N fixation; bacterial symbiosis with the rhizosphere; ammonium     formation; nitrification; denitrification.
5. Metal cycling: battle between chemical and biological pathways of metal transformations
    Structure and chemistry of minerals; metal speciation; Mn and Fe oxidation; metal oxidizers and anoxygenic phototrophs; chemical and bacterial metal reduction; bacterial mechanisms for detoxification; uptake of essential elements.
6. Sulfur cycle: the impact of sulfur-utilizing bacteria
    Sulfur chemistry; global sulfur cycle; sulfur oxidizers; sulfate reduction; microbial diversity in the sulfur cycle; oxidation of sulfur minerals.

VI. Organization of the Course

The course will consist of introductory lectures given by the instructors followed by review papers presented by the students. The course is designed to be a discussion between participants who will also read each paper assigned by the instructors. Participation is graded. Every student registered for the class is expected to read each paper carefully and have at least three questions to be addressed in class. Papers presentations should not exceed 30 minutes and will be followed by a 15 minute discussion. As a result, it is expected that two papers will be presented each lecture.
A term paper will be required from each student in the class. This paper should be a critical review of a published work of their choice, preferably in relation with the student’s own research. The term paper should not exceed 10 pages included figures and references.